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Two types of ANCA: p-ANCA (perinuclear), which is directed against the neutrophil enzyme myeloperoxidase (MPO), and c-ANCA (cytoplasmic), which is directed against the neutrophil proteinase 3 (PR3).
ANCA RPGN negative staining on immunofluorescence.
From https://www.vasculitis.org.uk/about-vasculitis/what-is-anca
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